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| LUZ,ANNY CAROLYNE DA; PRETTI,IRANY RODRIGUES; BATITUCCI,MARIA DO CARMO PIMENTEL. |
| ABSTRACT Functional genomic analyses require intact RNA; however, Passiflora edulis leaves are rich in secondary metabolites that interfere with RNA extraction primarily by promoting oxidative processes and by precipitating with nucleic acids. This study aimed to analyse three RNA extraction methods, Concert™ Plant RNA Reagent (Invitrogen, Carlsbad, CA, USA), TRIzol® Reagent (Invitrogen) and TRIzol® Reagent (Invitrogen)/ice -commercial products specifically designed to extract RNA, and to determine which method is the most effective for extracting RNA from the leaves of passion fruit plants. In contrast to the RNA extracted using the other 2 methods, the RNA extracted using TRIzol® Reagent (Invitrogen) did not have acceptable A260/A280 and A260/A230... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Passiflora edulis; RNA extraction; Concert™; TRIzol®; Low temperature. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452016000100226 |
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| Wrege,Marcos Silveira; Caramori,Paulo Henrique; Gonçalves,Sergio Luiz; Almeida,Wilson Paes de; Marur,Celso Jamil; Pires,José Ricoy; Yamaoka,Ruy Seiji. |
| Cotton is cultivated in the North and West of Parana State, southern Brazil, under conditions of climatic risk variable in space and time. Risks of temperature below 15ºC at the establishment period, daily average temperature below 20ºC at the stage of cotton boll opening, and soil water deficit for both plant establishment and flowering periods, were estimated to identify homogeneous zones with sowing periods of lower climatic risk. The time interval with adequate temperature, associated with minimum risks of the other factors and yield data from field experiments allowed the identification of seven distinct zones, with best sowing periods ranging from September 20 to November 20. Official credit to the farmers is conditioned by the Central Bank of Brazil... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cotton; Climatic risk; Planting dates; Zoning; Low temperature. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000100010 |
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| Boxaspen, Karin; Næss, Tore. |
| To verify if and to what extent egg and nauplii development of the salmon lice take place during winter, the development from egg to the copepodid stage at 2,3,4,5 and 10°C was examined. Newly extruded egg strings from a winter population of salmon lice were individually placed in 6 ml stagnant hatching systems. Initially, no significant differences in egg development time were found between these and larger aerated systems, though a tendency for less synchronised hatching of the total egg string was detected in the stagnant systems (difference< 12 h). In light versus dark conditions the time to hatching was significantly prolonged by darkness (10-15%). The use of small stagnant experimental units was a pronounced simplification of hatching methods... |
| Tipo: Article / Letter to the editor |
Palavras-chave: Salmon lice; Low temperature; Development of early life stages. |
| Ano: 2000 |
URL: http://www.repository.naturalis.nl/record/534414 |
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| Streck,Nereu Augusto; Schuh,Mariângela. |
| Vernalization is a process required by certain plant species, including lilies (Lilium spp.), to enter the reproductive phase, through an exposure to low, non-freezing temperatures. The objective of this study was to evaluate a nonlinear vernalization response function for the "Snow Queen" lily. An experiment was carried out in Santa Maria, RS, Brazil, to provide an independent data set to evaluate the performance of the model. Lily bulbs were vernalized at -0.5, 4.0, and 10ºC during two, four, six, and eight weeks. The daily vernalization rate (fvn) for each treatment was calculated with a beta function, and the effective vernalization days (VD) were calculated by accumulating fvn. The thermal time from plant emergence to visible buds at different VD... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Low temperature; Flowering; Bulb; Model; Plant development. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162005000200004 |
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